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Sample GSM550891 Query DataSets for GSM550891
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 42
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 63
gender: M
gold stage: Stage III
group: Severe
plateid: 2
batch: B
hyb date: 39321
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 1187.96143
AFFX-BioB-M_at 1752.55481
AFFX-BioB-3_at 1658.78918
AFFX-BioC-5_at 1124.27124
AFFX-BioC-3_at 827.8595
AFFX-BioDn-5_at 5542.0957
AFFX-BioDn-3_at 7787.68799
AFFX-CreX-5_at 11780.1875
AFFX-CreX-3_at 13356.63086
AFFX-DapX-5_at 29.0797
AFFX-DapX-M_at 14.26752
AFFX-DapX-3_at 68.24381
AFFX-LysX-5_at 17.33419
AFFX-LysX-M_at 9.5666
AFFX-LysX-3_at 2.64405
AFFX-PheX-5_at 23.29812
AFFX-PheX-M_at 21.99366
AFFX-PheX-3_at 31.71332
AFFX-ThrX-5_at 17.67199
AFFX-ThrX-M_at 13.76839

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550891.CEL.gz 4.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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