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Sample GSM550892 Query DataSets for GSM550892
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 43
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 57
gender: F
gold stage: Stage III
group: Severe
plateid: 2
batch: B
hyb date: 39321
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 771.73553
AFFX-BioB-M_at 1071.88245
AFFX-BioB-3_at 1030.97314
AFFX-BioC-5_at 735.8783
AFFX-BioC-3_at 527.26166
AFFX-BioDn-5_at 3731.93896
AFFX-BioDn-3_at 5368.2876
AFFX-CreX-5_at 8517.53223
AFFX-CreX-3_at 9593.68555
AFFX-DapX-5_at 32.42393
AFFX-DapX-M_at 13.98348
AFFX-DapX-3_at 41.99197
AFFX-LysX-5_at 9.29395
AFFX-LysX-M_at 10.51883
AFFX-LysX-3_at 16.77581
AFFX-PheX-5_at 16.39308
AFFX-PheX-M_at 13.54667
AFFX-PheX-3_at 30.39894
AFFX-ThrX-5_at 8.38179
AFFX-ThrX-M_at 16.13239

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550892.CEL.gz 5.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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