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Sample GSM550893 Query DataSets for GSM550893
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 44
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 69
gender: M
gold stage: Stage III
group: Severe
plateid: 2
batch: B
hyb date: 39321
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 582.14746
AFFX-BioB-M_at 854.02649
AFFX-BioB-3_at 758.48712
AFFX-BioC-5_at 579.40491
AFFX-BioC-3_at 406.41446
AFFX-BioDn-5_at 3281.48486
AFFX-BioDn-3_at 4933.77686
AFFX-CreX-5_at 8503.38672
AFFX-CreX-3_at 9183.37598
AFFX-DapX-5_at 54.11984
AFFX-DapX-M_at 4.83878
AFFX-DapX-3_at 25.38795
AFFX-LysX-5_at 8.93976
AFFX-LysX-M_at 14.08746
AFFX-LysX-3_at 7.97519
AFFX-PheX-5_at 13.21967
AFFX-PheX-M_at 17.81672
AFFX-PheX-3_at 30.30811
AFFX-ThrX-5_at 5.47401
AFFX-ThrX-M_at 20.12484

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550893.CEL.gz 5.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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