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Sample GSM550894 Query DataSets for GSM550894
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 56
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 60
gender: M
gold stage: Stage II
group: Moderate
plateid: 2
batch: B
hyb date: 39321
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 560.22803
AFFX-BioB-M_at 807.81775
AFFX-BioB-3_at 754.8761
AFFX-BioC-5_at 520.80237
AFFX-BioC-3_at 362.28433
AFFX-BioDn-5_at 3596.46216
AFFX-BioDn-3_at 5187.80029
AFFX-CreX-5_at 8432.62891
AFFX-CreX-3_at 9539.92578
AFFX-DapX-5_at 40.72857
AFFX-DapX-M_at 6.37781
AFFX-DapX-3_at 29.40126
AFFX-LysX-5_at 12.52699
AFFX-LysX-M_at 16.2263
AFFX-LysX-3_at 6.34836
AFFX-PheX-5_at 23.4889
AFFX-PheX-M_at 13.87319
AFFX-PheX-3_at 15.9448
AFFX-ThrX-5_at 3.32652
AFFX-ThrX-M_at 9.53311

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550894.CEL.gz 5.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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