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Sample GSM550896 Query DataSets for GSM550896
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 46
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 55
gender: M
gold stage: Stage III
group: Severe
plateid: 2
batch: B
hyb date: 39321
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 468.95657
AFFX-BioB-M_at 695.63483
AFFX-BioB-3_at 582.23895
AFFX-BioC-5_at 436.34402
AFFX-BioC-3_at 310.62286
AFFX-BioDn-5_at 3043.56201
AFFX-BioDn-3_at 4580.42627
AFFX-CreX-5_at 8204.92383
AFFX-CreX-3_at 9117.375
AFFX-DapX-5_at 53.89131
AFFX-DapX-M_at 8.46498
AFFX-DapX-3_at 26.53869
AFFX-LysX-5_at 15.5591
AFFX-LysX-M_at 7.0142
AFFX-LysX-3_at 3.154
AFFX-PheX-5_at 12.56525
AFFX-PheX-M_at 15.43229
AFFX-PheX-3_at 25.96471
AFFX-ThrX-5_at 15.59787
AFFX-ThrX-M_at 19.97248

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550896.CEL.gz 5.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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