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Sample GSM550898 Query DataSets for GSM550898
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 47
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 68
gender: M
gold stage: Stage III
group: Severe
plateid: 2
batch: B
hyb date: 39321
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 1007.47491
AFFX-BioB-M_at 1343.20471
AFFX-BioB-3_at 1298.16943
AFFX-BioC-5_at 1007.24683
AFFX-BioC-3_at 717.62323
AFFX-BioDn-5_at 3858.01025
AFFX-BioDn-3_at 5385.22266
AFFX-CreX-5_at 8101.70312
AFFX-CreX-3_at 9229.78711
AFFX-DapX-5_at 31.21466
AFFX-DapX-M_at 6.58467
AFFX-DapX-3_at 26.13539
AFFX-LysX-5_at 4.45349
AFFX-LysX-M_at 15.51359
AFFX-LysX-3_at 6.16941
AFFX-PheX-5_at 12.79358
AFFX-PheX-M_at 20.51219
AFFX-PheX-3_at 31.36077
AFFX-ThrX-5_at 2.77147
AFFX-ThrX-M_at 30.58268

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550898.CEL.gz 4.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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