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Sample GSM550902 Query DataSets for GSM550902
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 51
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 69
gender: F
gold stage: Stage III
group: Severe
plateid: 2
batch: B
hyb date: 39316
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 712.29773
AFFX-BioB-M_at 1030.22803
AFFX-BioB-3_at 954.79047
AFFX-BioC-5_at 709.37451
AFFX-BioC-3_at 500.2518
AFFX-BioDn-5_at 3635.92017
AFFX-BioDn-3_at 5156.20947
AFFX-CreX-5_at 8016.65088
AFFX-CreX-3_at 8655.49707
AFFX-DapX-5_at 41.05358
AFFX-DapX-M_at 8.70723
AFFX-DapX-3_at 22.4182
AFFX-LysX-5_at 5.28406
AFFX-LysX-M_at 13.82157
AFFX-LysX-3_at 4.48782
AFFX-PheX-5_at 15.29448
AFFX-PheX-M_at 13.68674
AFFX-PheX-3_at 24.15
AFFX-ThrX-5_at 8.39187
AFFX-ThrX-M_at 11.67674

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550902.CEL.gz 5.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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