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Sample GSM550903 Query DataSets for GSM550903
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 58
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 56
gender: F
gold stage: Stage II
group: Moderate
plateid: 2
batch: B
hyb date: 39321
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 571.85022
AFFX-BioB-M_at 837.09003
AFFX-BioB-3_at 747.40369
AFFX-BioC-5_at 558.41235
AFFX-BioC-3_at 386.51102
AFFX-BioDn-5_at 3627.02271
AFFX-BioDn-3_at 5037.42285
AFFX-CreX-5_at 8302.01758
AFFX-CreX-3_at 9038.92188
AFFX-DapX-5_at 54.67898
AFFX-DapX-M_at 3.31429
AFFX-DapX-3_at 22.7529
AFFX-LysX-5_at 13.03232
AFFX-LysX-M_at 13.66443
AFFX-LysX-3_at 7.51405
AFFX-PheX-5_at 13.10729
AFFX-PheX-M_at 17.11102
AFFX-PheX-3_at 19.38926
AFFX-ThrX-5_at 7.88021
AFFX-ThrX-M_at 25.41014

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550903.CEL.gz 5.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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