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Sample GSM550905 Query DataSets for GSM550905
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 59
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 64
gender: F
gold stage: Stage II
group: Moderate
plateid: 2
batch: B
hyb date: 39321
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 599.72986
AFFX-BioB-M_at 866.0235
AFFX-BioB-3_at 771.24231
AFFX-BioC-5_at 556.30438
AFFX-BioC-3_at 376.37479
AFFX-BioDn-5_at 3479.53711
AFFX-BioDn-3_at 5476.87354
AFFX-CreX-5_at 8576.35254
AFFX-CreX-3_at 9395
AFFX-DapX-5_at 41.76752
AFFX-DapX-M_at 9.27111
AFFX-DapX-3_at 38.37855
AFFX-LysX-5_at 15.60721
AFFX-LysX-M_at 8.41137
AFFX-LysX-3_at 3.8117
AFFX-PheX-5_at 12.14079
AFFX-PheX-M_at 23.60484
AFFX-PheX-3_at 18.85742
AFFX-ThrX-5_at 17.03367
AFFX-ThrX-M_at 12.56709

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550905.CEL.gz 5.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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