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Sample GSM550906 Query DataSets for GSM550906
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 60
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 72
gender: F
gold stage: Stage II
group: Moderate
plateid: 2
batch: B
hyb date: 39316
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 599.63824
AFFX-BioB-M_at 914.33014
AFFX-BioB-3_at 830.88306
AFFX-BioC-5_at 599.1369
AFFX-BioC-3_at 430.27597
AFFX-BioDn-5_at 3787.95728
AFFX-BioDn-3_at 5674.84424
AFFX-CreX-5_at 8641.81348
AFFX-CreX-3_at 9882.68164
AFFX-DapX-5_at 37.34599
AFFX-DapX-M_at 6.16882
AFFX-DapX-3_at 22.2759
AFFX-LysX-5_at 12.49103
AFFX-LysX-M_at 9.14256
AFFX-LysX-3_at 11.34875
AFFX-PheX-5_at 17.93614
AFFX-PheX-M_at 11.61474
AFFX-PheX-3_at 30.26068
AFFX-ThrX-5_at 5.92709
AFFX-ThrX-M_at 16.21018

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550906.CEL.gz 5.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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