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Sample GSM550907 Query DataSets for GSM550907
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 61
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 72
gender: M
gold stage: Stage II
group: Moderate
plateid: 2
batch: B
hyb date: 39315
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 998.61804
AFFX-BioB-M_at 1428.22095
AFFX-BioB-3_at 1298.28076
AFFX-BioC-5_at 1000.66663
AFFX-BioC-3_at 726.81287
AFFX-BioDn-5_at 4794.83154
AFFX-BioDn-3_at 6257.80029
AFFX-CreX-5_at 9100.93848
AFFX-CreX-3_at 10230.5293
AFFX-DapX-5_at 35.15974
AFFX-DapX-M_at 7.23184
AFFX-DapX-3_at 25.85495
AFFX-LysX-5_at 10.64457
AFFX-LysX-M_at 14.05035
AFFX-LysX-3_at 12.69756
AFFX-PheX-5_at 16.21852
AFFX-PheX-M_at 13.89416
AFFX-PheX-3_at 24.58995
AFFX-ThrX-5_at 5.04621
AFFX-ThrX-M_at 5.91005

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550907.CEL.gz 5.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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