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Sample GSM550908 Query DataSets for GSM550908
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 53
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 70
gender: F
gold stage: Stage III
group: Severe
plateid: 2
batch: B
hyb date: 39321
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 1224.54956
AFFX-BioB-M_at 1597.59131
AFFX-BioB-3_at 1522.26233
AFFX-BioC-5_at 1209.04553
AFFX-BioC-3_at 826.74579
AFFX-BioDn-5_at 5035.27051
AFFX-BioDn-3_at 6908.14307
AFFX-CreX-5_at 9944.74707
AFFX-CreX-3_at 11444.83105
AFFX-DapX-5_at 29.34415
AFFX-DapX-M_at 7.44541
AFFX-DapX-3_at 27.98189
AFFX-LysX-5_at 16.8584
AFFX-LysX-M_at 6.93318
AFFX-LysX-3_at 15.40266
AFFX-PheX-5_at 9.88233
AFFX-PheX-M_at 6.82204
AFFX-PheX-3_at 38.61035
AFFX-ThrX-5_at 6.09592
AFFX-ThrX-M_at 2.86178

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550908.CEL.gz 4.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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