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Sample GSM550909 Query DataSets for GSM550909
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 62
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 63
gender: M
gold stage: Stage II
group: Moderate
plateid: 2
batch: B
hyb date: 39316
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 686.06628
AFFX-BioB-M_at 1032.0835
AFFX-BioB-3_at 904.95392
AFFX-BioC-5_at 648.03467
AFFX-BioC-3_at 472.82224
AFFX-BioDn-5_at 3564.46899
AFFX-BioDn-3_at 5103.12598
AFFX-CreX-5_at 7799.29688
AFFX-CreX-3_at 8615.58301
AFFX-DapX-5_at 50.4131
AFFX-DapX-M_at 8.88266
AFFX-DapX-3_at 43.59708
AFFX-LysX-5_at 10.56494
AFFX-LysX-M_at 10.95728
AFFX-LysX-3_at 8.65573
AFFX-PheX-5_at 11.71233
AFFX-PheX-M_at 11.37388
AFFX-PheX-3_at 23.30092
AFFX-ThrX-5_at 9.45322
AFFX-ThrX-M_at 16.98637

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550909.CEL.gz 5.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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