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Sample GSM550910 Query DataSets for GSM550910
Status Public on Jun 08, 2010
Title Sputum from Stage IV Severe, biological replicate 11
Sample type RNA
 
Source name Sputum from COPD Stage IV Severe
Organism Homo sapiens
Characteristics age: 55
gender: F
gold stage: Stage IV
group: Severe
plateid: 2
batch: B
hyb date: 39315
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage IV Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 745.32574
AFFX-BioB-M_at 1055.8385
AFFX-BioB-3_at 930.51923
AFFX-BioC-5_at 737.72974
AFFX-BioC-3_at 545.6347
AFFX-BioDn-5_at 3830.40967
AFFX-BioDn-3_at 5207.48438
AFFX-CreX-5_at 8189.44043
AFFX-CreX-3_at 8710.91797
AFFX-DapX-5_at 68.71165
AFFX-DapX-M_at 4.63857
AFFX-DapX-3_at 22.99932
AFFX-LysX-5_at 9.59321
AFFX-LysX-M_at 15.24441
AFFX-LysX-3_at 2.48615
AFFX-PheX-5_at 23.22339
AFFX-PheX-M_at 16.65965
AFFX-PheX-3_at 30.93653
AFFX-ThrX-5_at 14.12002
AFFX-ThrX-M_at 19.08667

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550910.CEL.gz 5.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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