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Sample GSM550913 Query DataSets for GSM550913
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 65
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 53
gender: M
gold stage: Stage II
group: Moderate
plateid: 2
batch: B
hyb date: 39321
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 452.29724
AFFX-BioB-M_at 670.70343
AFFX-BioB-3_at 607.12909
AFFX-BioC-5_at 443.47205
AFFX-BioC-3_at 293.76071
AFFX-BioDn-5_at 2989.34937
AFFX-BioDn-3_at 4632.36426
AFFX-CreX-5_at 7881.96973
AFFX-CreX-3_at 8796.58008
AFFX-DapX-5_at 41.03612
AFFX-DapX-M_at 5.95831
AFFX-DapX-3_at 26.15203
AFFX-LysX-5_at 21.02628
AFFX-LysX-M_at 14.23927
AFFX-LysX-3_at 4.05236
AFFX-PheX-5_at 16.43804
AFFX-PheX-M_at 7.65687
AFFX-PheX-3_at 18.79929
AFFX-ThrX-5_at 24.27003
AFFX-ThrX-M_at 9.94744

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550913.CEL.gz 5.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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