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Sample GSM550915 Query DataSets for GSM550915
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 66
Sample type RNA
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 73
gender: M
gold stage: Stage II
group: Moderate
plateid: 2
batch: B
hyb date: 39316
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
AFFX-BioB-5_at 589.33704
AFFX-BioB-M_at 890.39038
AFFX-BioB-3_at 779.19507
AFFX-BioC-5_at 548.91248
AFFX-BioC-3_at 394.8685
AFFX-BioDn-5_at 3378.29639
AFFX-BioDn-3_at 5241.75
AFFX-CreX-5_at 8449.18262
AFFX-CreX-3_at 9535.3916
AFFX-DapX-5_at 41.9399
AFFX-DapX-M_at 7.36805
AFFX-DapX-3_at 19.817
AFFX-LysX-5_at 11.46901
AFFX-LysX-M_at 2.55891
AFFX-LysX-3_at 6.57777
AFFX-PheX-5_at 14.33647
AFFX-PheX-M_at 14.30244
AFFX-PheX-3_at 27.41701
AFFX-ThrX-5_at 10.94059
AFFX-ThrX-M_at 15.38519

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.

Supplementary file Size Download File type/resource
GSM550915.CEL.gz 5.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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