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Sample GSM550918 Query DataSets for GSM550918
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 56
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 60
gender: F
gold stage: Stage III
group: Severe
plateid: 2
batch: B
hyb date: 39316
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 580.75916
AFFX-BioB-M_at 832.68848
AFFX-BioB-3_at 733.0014
AFFX-BioC-5_at 559.06885
AFFX-BioC-3_at 394.97861
AFFX-BioDn-5_at 3383.46704
AFFX-BioDn-3_at 4762.04639
AFFX-CreX-5_at 8202.12012
AFFX-CreX-3_at 8977.14062
AFFX-DapX-5_at 38.60818
AFFX-DapX-M_at 5.37343
AFFX-DapX-3_at 20.93161
AFFX-LysX-5_at 13.08711
AFFX-LysX-M_at 8.1357
AFFX-LysX-3_at 2.19517
AFFX-PheX-5_at 22.78612
AFFX-PheX-M_at 11.29992
AFFX-PheX-3_at 21.72498
AFFX-ThrX-5_at 17.72409
AFFX-ThrX-M_at 12.94291

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550918.CEL.gz 5.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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