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Sample GSM550919 Query DataSets for GSM550919
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 68
Sample type RNA
 
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 66
gender: M
gold stage: Stage II
group: Moderate
plateid: 2
batch: B
hyb date: 39316
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 621.05359
AFFX-BioB-M_at 939.6214
AFFX-BioB-3_at 874.11304
AFFX-BioC-5_at 633.3056
AFFX-BioC-3_at 448.03525
AFFX-BioDn-5_at 3682.13818
AFFX-BioDn-3_at 5065.90625
AFFX-CreX-5_at 8353.1709
AFFX-CreX-3_at 9077.37988
AFFX-DapX-5_at 33.25632
AFFX-DapX-M_at 8.60964
AFFX-DapX-3_at 32.019
AFFX-LysX-5_at 15.16189
AFFX-LysX-M_at 16.68063
AFFX-LysX-3_at 9.47902
AFFX-PheX-5_at 17.79491
AFFX-PheX-M_at 16.43377
AFFX-PheX-3_at 33.06387
AFFX-ThrX-5_at 10.68991
AFFX-ThrX-M_at 20.14743

Total number of rows: 54675

Table truncated, full table size 1071 Kbytes.




Supplementary file Size Download File type/resource
GSM550919.CEL.gz 5.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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