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Sample GSM550920 Query DataSets for GSM550920
Status Public on Jun 08, 2010
Title Sputum from Stage IV Severe, biological replicate 13
Sample type RNA
 
Source name Sputum from COPD Stage IV Severe
Organism Homo sapiens
Characteristics age: 75
gender: M
gold stage: Stage IV
group: Severe
plateid: 2
batch: C
hyb date: 39322
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage IV Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 657.95801
AFFX-BioB-M_at 980.02808
AFFX-BioB-3_at 834.84778
AFFX-BioC-5_at 1933.92468
AFFX-BioC-3_at 1516.80481
AFFX-BioDn-5_at 3424.6665
AFFX-BioDn-3_at 5373.81104
AFFX-CreX-5_at 8947.52637
AFFX-CreX-3_at 9692.51074
AFFX-DapX-5_at 54.46418
AFFX-DapX-M_at 7.10823
AFFX-DapX-3_at 18.27187
AFFX-LysX-5_at 8.54797
AFFX-LysX-M_at 4.76729
AFFX-LysX-3_at 5.96942
AFFX-PheX-5_at 21.85709
AFFX-PheX-M_at 14.33424
AFFX-PheX-3_at 19.52373
AFFX-ThrX-5_at 17.73693
AFFX-ThrX-M_at 22.14521

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550920.CEL.gz 5.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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