|
| Status |
Public on Jun 08, 2010 |
| Title |
Sputum from Stage III Severe, biological replicate 57 |
| Sample type |
RNA |
| |
|
| Source name |
Sputum from COPD Stage III Severe
|
| Organism |
Homo sapiens |
| Characteristics |
age: 70
gender: M
gold stage: Stage III
group: Severe
plateid: 2
batch: B
hyb date: 39315
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
|
| |
|
| Hybridization protocol |
Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
|
| Description |
Gene expression data from COPD Stage III Severe
|
| Data processing |
The data was analysed with Rosetta Resolver version 5.0
|
| |
|
| Submission date |
Jun 04, 2010 |
| Last update date |
Jun 07, 2010 |
| Contact name |
steve matthew fox |
| E-mail(s) |
steven.m.fox@gsk.com
|
| Phone |
01438 763226
|
| Organization name |
GlaxoSmithKline
|
| Department |
Statistics
|
| Street address |
Gunnels Wood Road
|
| City |
Stevenage |
| ZIP/Postal code |
SG1 2NY |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE22148 |
Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers |
|
