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Sample GSM550922 Query DataSets for GSM550922
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 57
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 70
gender: M
gold stage: Stage III
group: Severe
plateid: 2
batch: B
hyb date: 39315
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 600.22992
AFFX-BioB-M_at 898.45148
AFFX-BioB-3_at 787.14929
AFFX-BioC-5_at 584.53497
AFFX-BioC-3_at 423.34201
AFFX-BioDn-5_at 3706.19312
AFFX-BioDn-3_at 5306.04053
AFFX-CreX-5_at 8720.86816
AFFX-CreX-3_at 9605.14062
AFFX-DapX-5_at 40.69083
AFFX-DapX-M_at 9.41195
AFFX-DapX-3_at 25.1568
AFFX-LysX-5_at 14.46658
AFFX-LysX-M_at 5.43067
AFFX-LysX-3_at 6.09154
AFFX-PheX-5_at 22.28559
AFFX-PheX-M_at 18.13397
AFFX-PheX-3_at 19.95394
AFFX-ThrX-5_at 13.47869
AFFX-ThrX-M_at 14.52574

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550922.CEL.gz 5.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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