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Sample GSM550923 Query DataSets for GSM550923
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 58
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 62
gender: M
gold stage: Stage III
group: Severe
plateid: 2
batch: B
hyb date: 39321
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 1239.13757
AFFX-BioB-M_at 1773.26709
AFFX-BioB-3_at 1617.11255
AFFX-BioC-5_at 1313.75049
AFFX-BioC-3_at 891.71155
AFFX-BioDn-5_at 4815.0542
AFFX-BioDn-3_at 6463.41357
AFFX-CreX-5_at 9034.60156
AFFX-CreX-3_at 10531.41895
AFFX-DapX-5_at 35.03168
AFFX-DapX-M_at 9.06209
AFFX-DapX-3_at 25.65034
AFFX-LysX-5_at 19.246
AFFX-LysX-M_at 46.59053
AFFX-LysX-3_at 11.30908
AFFX-PheX-5_at 27.02596
AFFX-PheX-M_at 28.30508
AFFX-PheX-3_at 31.96975
AFFX-ThrX-5_at 8.1723
AFFX-ThrX-M_at 30.10808

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550923.CEL.gz 4.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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