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Sample GSM550925 Query DataSets for GSM550925
Status Public on Jun 08, 2010
Title Sputum from Stage III Severe, biological replicate 59
Sample type RNA
 
Source name Sputum from COPD Stage III Severe
Organism Homo sapiens
Characteristics age: 69
gender: M
gold stage: Stage III
group: Severe
plateid: 2
batch: B
hyb date: 39316
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
 
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage III Severe
Data processing The data was analysed with Rosetta Resolver version 5.0
 
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
ID_REF
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 702.4209
AFFX-BioB-M_at 1131.15149
AFFX-BioB-3_at 1017.19446
AFFX-BioC-5_at 737.80371
AFFX-BioC-3_at 542.84406
AFFX-BioDn-5_at 4052.00244
AFFX-BioDn-3_at 5518.43848
AFFX-CreX-5_at 8736.99414
AFFX-CreX-3_at 9506.82031
AFFX-DapX-5_at 46.27016
AFFX-DapX-M_at 6.86296
AFFX-DapX-3_at 32.10828
AFFX-LysX-5_at 12.54207
AFFX-LysX-M_at 18.44114
AFFX-LysX-3_at 9.31248
AFFX-PheX-5_at 20.24397
AFFX-PheX-M_at 12.722
AFFX-PheX-3_at 31.35144
AFFX-ThrX-5_at 11.34482
AFFX-ThrX-M_at 26.03599

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.




Supplementary file Size Download File type/resource
GSM550925.CEL.gz 5.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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