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Sample GSM550926 Query DataSets for GSM550926
Status Public on Jun 08, 2010
Title Sputum from Stage II Moderate, biological replicate 71
Sample type RNA
Source name Sputum from COPD Stage II Moderate
Organism Homo sapiens
Characteristics age: 57
gender: F
gold stage: Stage II
group: Moderate
plateid: 2
batch: B
hyb date: 39315
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol NuGEN Ovation FLv2 methodology using NuGEN Ovation RNA amplification v2 including C1 spike with Agencourt RNAClean SPIA clean up. FLv2 biotin fragmentation and labelling.
Hybridization protocol Following fragmentation, 3.75 ug of cDNA were hybridized for 16 hr at 45C on GeneChip HG-U133_plus_2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
Description Gene expression data from COPD Stage II Moderate
Data processing The data was analysed with Rosetta Resolver version 5.0
Submission date Jun 04, 2010
Last update date Jun 07, 2010
Contact name steve matthew fox
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
Platform ID GPL570
Series (1)
GSE22148 Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers

Data table header descriptions
VALUE Rosetta Resolver 5.0 Nomalised Intensity

Data table
AFFX-BioB-5_at 610.1004
AFFX-BioB-M_at 918.74097
AFFX-BioB-3_at 783.88159
AFFX-BioC-5_at 599.15283
AFFX-BioC-3_at 419.2789
AFFX-BioDn-5_at 3793.31323
AFFX-BioDn-3_at 5356.17676
AFFX-CreX-5_at 8856.30078
AFFX-CreX-3_at 9911.7334
AFFX-DapX-5_at 45.23355
AFFX-DapX-M_at 10.19128
AFFX-DapX-3_at 16.44499
AFFX-LysX-5_at 13.49795
AFFX-LysX-M_at 11.69572
AFFX-LysX-3_at 9.48163
AFFX-PheX-5_at 19.13236
AFFX-PheX-M_at 20.95627
AFFX-PheX-3_at 29.60199
AFFX-ThrX-5_at 23.92868
AFFX-ThrX-M_at 13.1725

Total number of rows: 54675

Table truncated, full table size 1070 Kbytes.

Supplementary file Size Download File type/resource
GSM550926.CEL.gz 5.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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