|
| Status |
Public on Jun 17, 2010 |
| Title |
T7-SAGE transcriptome of end bud epithelial cells of embryonic mouse salivary glands |
| Sample type |
SAGE |
| Anchor |
NlaIII
|
| Tag Count |
22244 |
| Tag Length |
10 |
| |
|
| Source name |
Embryonic submandibular salivary gland
|
| Organism |
Mus musculus |
| Characteristics |
cell type: laser microdissected epithelial cells from end bud of embryonic salivary gland
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Protocol as published in: Sakai T, Larsen M, Yamada KM. Microanalysis of gene expression in tissues using T7-SAGE: serial analysis of gene expression after high-fidelity T7-based RNA amplification. Curr Protoc Cell Biol. 2002 Nov;Chapter 19:Unit 19.3.
|
| |
|
| Description |
Mus musculus embryonic submandibular salivary glands from embryonic day 12.5 embryos were laser microdissected to isolate epithelial cells from regions adjacent to forming clefts or from the peripheral end bud region. The isolated region-specific samples were then subjected to T7-SAGE transcriptome analysis.
|
| Data processing |
SAGE 2000 software from http://www.sagenet.org
|
| |
|
| Submission date |
Jun 14, 2010 |
| Last update date |
Jun 16, 2010 |
| Contact name |
Takayoshi Sakai |
| E-mail(s) |
sakai@dent.osaka-u.ac.jp
|
| Phone |
81-6-6879-2275
|
| Fax |
81-6-6879-2279
|
| Organization name |
Osaka Univ Grad Sch Dent
|
| Department |
Oral-Facial Disorders
|
| Street address |
1-8 Yamadaoka
|
| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
5650871 |
| Country |
Japan |
| |
|
| Platform ID |
GPL11 |
| Series (1) |
| GSE22374 |
T7-SAGE transcriptome of cleft versus end bud epithelial cells of embryonic mouse salivary glands |
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