|
| Status |
Public on Jun 16, 2010 |
| Title |
Homozygous Chd7 Null replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse ES cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C3HeB/FeJ
cell type: mouse ES cells
genotype/variation: Homozygous Chd7 Null
|
| Growth protocol |
ES cells were cultured using feeder MEFs according to standard procotols.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Trizol. The ratio of ES to feeder cells was estimated at 5:1.
|
| Label |
biotin
|
| Label protocol |
RNA was labeled and hybridized to Illumina Mouse Ref-8 v2 Expression BeadChIP microarrays according to the manufacturer’s instructions
|
| |
|
| Hybridization protocol |
RNA was labeled and hybridized to Illumina Mouse Ref-8 v2 Expression BeadChIP microarrays according to the manufacturer’s instructions
|
| Scan protocol |
Illumina Mouse Ref-8 v2 Expression BeadChIP microarrays were scanned according to the manufacturer’s instructions.
|
| Description |
replicate 2
|
| Data processing |
Raw data were background subtracted and quantile normalized using Illumina Bead Studio software version 3
Unnormalized data are signal intensity with background subtracted; normailized data are signal intensity with background subtracted and quantile normalized using Illumina Bead Studio software
|
| |
|
| Submission date |
Jun 14, 2010 |
| Last update date |
Jun 15, 2010 |
| Contact name |
Mike Paul Schnetz |
| E-mail(s) |
mps35@case.edu
|
| Phone |
216.368.1256
|
| Organization name |
Case Western Reserve University
|
| Department |
Genetics
|
| Lab |
Scacheri
|
| Street address |
10900 Eulid Ave
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44106 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE22341 |
CHD7 targets active gene enhancer elements to modulate ES cell-specific gene expression. |
|
