|
| Status |
Public on Nov 02, 2010 |
| Title |
LV-T350A&si-EZH2-2 |
| Sample type |
RNA |
| |
|
| Source name |
5411204047_D
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: BJ
transfection: si-EZH2 plus RNAi resistant lentivirus expressed T350A mutant
|
| Treatment protocol |
Cells were transducted by lentivirus for 72h, followed by si-RNA transfection for 60 h.
|
| Growth protocol |
BJ cells were grown in DMEM medium supplemented with 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the Qiagen RNeasy kit (Qiagen Inc).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Jun 17, 2010 |
| Last update date |
Nov 02, 2010 |
| Contact name |
Shuai Chen |
| E-mail(s) |
chenx495@umn.edu
|
| Organization name |
University of Minnesota
|
| Department |
Cancer Center
|
| Street address |
MMC 806, 420 Delaware Street S.E.
|
| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE22427 |
Effect of T350 phosphorylation on gene repression activity of EZH2 in BJ cells |
|
