|
| Status |
Public on Sep 15, 2021 |
| Title |
CM/miR-126Δ/Δ-5 |
| Sample type |
SRA |
| |
|
| Source name |
bone morrow
|
| Organism |
Mus musculus |
| Characteristics |
cell type: LSK from mice bone morrow cells
genotype/variation: CM/miR-126delta/delta
treatment: poly(I;C)
Stage: pre-leukemic [6 weeks after poly(I;C) induction]
molecule concentration: 18.2ng/ul
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Library contruction was used KAPA RNA HyperPrep Kit with RiboErase (HMR) according to manufacturer's protocol.
Depletion of ribosomal RNA (rRNA) and the rapid construction of stranded RNA-Seq libraries
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
Sample_37518_710_S4_Group_C
|
| Data processing |
Base calling was done using Illumina RTA and bcl2fastq v2.18.0.12
Sequences were aligned to mm10 using Tophat2 with default settings
Gene expression level of Ensembl GRCm38 coding genes were quantified using HTSeq v0.6.1p1
Genome_build: mm10
Supplementary_files_format_and_content: tab delimited text file gene expression counts
|
| |
|
| Submission date |
Sep 13, 2021 |
| Last update date |
Sep 15, 2021 |
| Contact name |
wei chen |
| E-mail(s) |
weichen2@coh.org
|
| Organization name |
City of Hope National Medical Center
|
| Department |
Cancer Biology-BRI
|
| Street address |
1500 E Duarte Rd
|
| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010 |
| Country |
USA |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE184015 |
Targeting miR-126 in inv(16) acute myeloid leukemia inhibits leukemia development and leukemia stem cell maintenance |
|
| Relations |
| BioSample |
SAMN21412389 |
| SRA |
SRX12155382 |
