|
Status |
Public on Sep 16, 2021 |
Title |
pEXO_monocyte_01 |
Sample type |
SRA |
|
|
Source name |
monocyte
|
Organism |
Homo sapiens |
Characteristics |
cell type: monocyte source: Buffy coat treatment: Treated with pEXO
|
Treatment protocol |
isolated monocytes were treated in the presence or absence of 20μg/ml pEXO
|
Growth protocol |
Primary monocytes were isolated by Ficoll-Paque density gradient centrifugation. Monocyte isolated by positive immunomagnetic separation. Culture in RPMI1640 + 10% FBS
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted by Trizol The libraries were prepared according to Illumina TruSeq® RNA sample preparation Kit.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
MGISEQ-2000RS |
|
|
Data processing |
Low-quality reads were removed by fastp with default parameters Remaining clean reads were align to the Human genome 38 (hg38) by Hisat2 with default parameters Raw gene counts were generated by featurecount and then normalized by DEseq2 Genome_build: hg38 Supplementary_files_format_and_content: Matrix tables with raw gene counts or normalized gene counts for every gene and every sample
|
|
|
Submission date |
Sep 13, 2021 |
Last update date |
Sep 16, 2021 |
Contact name |
Jianlin Li |
Organization name |
The university of Hong Kong
|
Street address |
L 7-08, Department of Obstetrics and Gynecology Laboratory block, 21th Sassoon Road, Hong Kong
|
City |
Hong Kong |
ZIP/Postal code |
999077 |
Country |
Hong Kong |
|
|
Platform ID |
GPL30209 |
Series (2) |
GSE184020 |
Human placental exosomes induce maternal systemic immune tolerance in a monocyte-dependent manner |
GSE184195 |
Placental exosomes |
|
Relations |
BioSample |
SAMN21412623 |
SRA |
SRX12156525 |