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Sample GSM5576039 Query DataSets for GSM5576039
Status Public on Sep 16, 2021
Title pEXO_monocyte_02
Sample type SRA
 
Source name monocyte
Organism Homo sapiens
Characteristics cell type: monocyte
source: Buffy coat
treatment: Treated with pEXO
Treatment protocol isolated monocytes were treated in the presence or absence of 20μg/ml pEXO
Growth protocol Primary monocytes were isolated by Ficoll-Paque density gradient centrifugation. Monocyte isolated by positive immunomagnetic separation. Culture in RPMI1640 + 10% FBS
Extracted molecule total RNA
Extraction protocol Total RNA was extracted by Trizol
The libraries were prepared according to Illumina TruSeq® RNA sample preparation Kit.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model MGISEQ-2000RS
 
Data processing Low-quality reads were removed by fastp with default parameters
Remaining clean reads were align to the Human genome 38 (hg38) by Hisat2 with default parameters
Raw gene counts were generated by featurecount and then normalized by DEseq2
Genome_build: hg38
Supplementary_files_format_and_content: Matrix tables with raw gene counts or normalized gene counts for every gene and every sample
 
Submission date Sep 13, 2021
Last update date Sep 16, 2021
Contact name Jianlin Li
Organization name The university of Hong Kong
Street address L 7-08, Department of Obstetrics and Gynecology Laboratory block, 21th Sassoon Road, Hong Kong
City Hong Kong
ZIP/Postal code 999077
Country Hong Kong
 
Platform ID GPL30209
Series (2)
GSE184020 Human placental exosomes induce maternal systemic immune tolerance in a monocyte-dependent manner
GSE184195 Placental exosomes
Relations
BioSample SAMN21412624
SRA SRX12156526

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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