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Sample GSM5580100 Query DataSets for GSM5580100
Status Public on Sep 16, 2021
Title Contorl_macrophage_02
Sample type SRA
 
Source name human monocyte-derived macrophage
Organism Homo sapiens
Characteristics source: buffy coat
treatment with pexo: untreated
induction: M-CSF
Treatment protocol Macrophages were prepared by treating monocytes with 50 ng/ml MCSF for7 days and at Day 8 treated in the presence or absence ofpEXO (20 μg/ml)
Growth protocol Primary monocytes were isolated by Ficoll-Paque density gradient centrifugation. Monocyte isolated by positive immunomagnetic separation. Culture in RPMI1640 + 10% FBS
Extracted molecule total RNA
Extraction protocol Total RNA was extracted by RNA spin column
The libraries were prepared according to Illumina TruSeq® RNA sample preparation Kit.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Data processing Low-quality reads were removed by fastp with default parameters
Remaining clean reads were align to the Human genome 38 (hg38) by Hisat2 with default parameters
Raw gene counts were generated by featurecount and then normalized by DEseq2
Genome_build: hg38
Supplementary_files_format_and_content: Matrix table with normalized gene counts for every gene and every sample
 
Submission date Sep 15, 2021
Last update date Sep 16, 2021
Contact name Jianlin Li
Organization name The university of Hong Kong
Street address L 7-08, Department of Obstetrics and Gynecology Laboratory block, 21th Sassoon Road, Hong Kong
City Hong Kong
ZIP/Postal code 999077
Country Hong Kong
 
Platform ID GPL11154
Series (2)
GSE184194 Placental exosomes polarize human monocyte-derived macrophages to a decidual M2-like macrophage phenotype
GSE184195 Placental exosomes
Relations
BioSample SAMN21442170
SRA SRX12195248

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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