|
| Status |
Public on Jun 24, 2010 |
| Title |
Control_3 |
| Sample type |
RNA |
| |
|
| Source name |
Alveolar macrophages
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Alveolar macrophages from broncholaveolar lavage
disease state: normal
|
| Growth protocol |
Alveolar macrophages (AM) were purified by adherence (2h) from bronchoalveolar lavage (BAL), giging purity of > 98%
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using a silica gel-based Rneasy spin column protocol (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Probe labelling was done at the McGill University and Genome Quebec Innovation Centre (www.genomequebec.mcgill.ca) and was performed in parallele for control and asthmatic samples
|
| |
|
| Hybridization protocol |
Hybridation was done at the McGill University and Genome Quebec Innovation Centre (www.genomequebec.mcgill.ca) and was performed in parallele for control and asthmatic samples
|
| Scan protocol |
Scanning was done using a GeneArray Scanner at the McGill University and Genome Quebec Innovation Centre (www.genomequebec.mcgill.ca) and was performed in parallele for control and asthmatic samples
|
| Description |
Gene expression data from non allergic non asthmatic subject, without any stimulation. BAL was done at the same time of the day for each subject and only if he (she) did not experience inflammatory or allergen response.
|
| Data processing |
The scanned images were analyzed using the Microarray Analysis Suite 5.0 (MAS5, Affymetrix) and the raw image fils obtained (.CEL) were used for statistical analyses. Affy and Limma packages in Bioconductor (www.bioconductor.org) using R2.8.1 were used to perform quality tests, RMA normalization and Smyth's moderated t-test.
|
| |
|
| Submission date |
Jun 23, 2010 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Catherine Laprise |
| Organization name |
Université du Québec à Chicoutimi
|
| Street address |
555 Boul. Université
|
| City |
Chicoutimi |
| State/province |
Qc |
| ZIP/Postal code |
G7H 2B1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE22528 |
Gene expression pattern of alveolar macrophages of allergic asthmatics in comparison with control subjects |
|
| Relations |
| Reanalyzed by |
GSE86363 |
