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Sample GSM559374 Query DataSets for GSM559374
Status Public on Jun 24, 2010
Title Control_4
Sample type RNA
 
Source name Alveolar macrophages
Organism Homo sapiens
Characteristics cell type: Alveolar macrophages from broncholaveolar lavage
disease state: normal
Growth protocol Alveolar macrophages (AM) were purified by adherence (2h) from bronchoalveolar lavage (BAL), giging purity of > 98%
Extracted molecule total RNA
Extraction protocol RNA was extracted using a silica gel-based Rneasy spin column protocol (Qiagen)
Label biotin
Label protocol Probe labelling was done at the McGill University and Genome Quebec Innovation Centre (www.genomequebec.mcgill.ca) and was performed in parallele for control and asthmatic samples
 
Hybridization protocol Hybridation was done at the McGill University and Genome Quebec Innovation Centre (www.genomequebec.mcgill.ca) and was performed in parallele for control and asthmatic samples
Scan protocol Scanning was done using a GeneArray Scanner at the McGill University and Genome Quebec Innovation Centre (www.genomequebec.mcgill.ca) and was performed in parallele for control and asthmatic samples
Description Gene expression data from non allergic non asthmatic subject, without any stimulation. BAL was done at the same time of the day for each subject and only if he (she) did not experience inflammatory or allergen response.
Data processing The scanned images were analyzed using the Microarray Analysis Suite 5.0 (MAS5, Affymetrix) and the raw image fils obtained (.CEL) were used for statistical analyses. Affy and Limma packages in Bioconductor (www.bioconductor.org) using R2.8.1 were used to perform quality tests, RMA normalization and Smyth's moderated t-test.
 
Submission date Jun 23, 2010
Last update date Sep 01, 2016
Contact name Catherine Laprise
Organization name Université du Québec à Chicoutimi
Street address 555 Boul. Université
City Chicoutimi
State/province Qc
ZIP/Postal code G7H 2B1
Country Canada
 
Platform ID GPL96
Series (1)
GSE22528 Gene expression pattern of alveolar macrophages of allergic asthmatics in comparison with control subjects
Relations
Reanalyzed by GSE86363

Data table header descriptions
ID_REF
VALUE Signal intensity after RMA normalization (log2)

Data table
ID_REF VALUE
1007_s_at 6.853409526
1053_at 6.64897258
117_at 7.997813824
121_at 8.820172753
1255_g_at 3.462914695
1294_at 7.260581175
1316_at 4.669632098
1320_at 4.583023511
1405_i_at 4.592557607
1431_at 3.262366962
1438_at 6.023876382
1487_at 7.356837064
1494_f_at 6.037478585
1598_g_at 7.634764925
160020_at 7.374540305
1729_at 7.673232545
177_at 4.843119204
1773_at 4.93198797
179_at 8.505285348
1861_at 5.5014056

Total number of rows: 22283

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM559374.CEL.gz 3.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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