|
Status |
Public on Jun 24, 2010 |
Title |
Sirt1KO#2 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Sirt1KO
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 gender: Male tissue: hippocampus
|
Extracted molecule |
total RNA |
Extraction protocol |
Total hippocampul RNA was isolated using TRIzol (Invitrogen) followed by purification over a glass fiber filter column (mirVana; Ambion), according to manufacturers' instructions. RNA quality was assessed using an Agilent BioAnalyzer 2100.
|
Label |
Cy3
|
Label protocol |
RNA samples were sent to Exiqon A/S (Vedbaek, Denmark) where they were labeled using the miRCURY Hy3/Hy5 labeling kit.
|
|
|
Channel 2 |
Source name |
common reference (comprised of an equal mixture of all 4 experimental samples)
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 gender: Male tissue: hippocampus
|
Extracted molecule |
total RNA |
Extraction protocol |
Total hippocampul RNA was isolated using TRIzol (Invitrogen) followed by purification over a glass fiber filter column (mirVana; Ambion), according to manufacturers' instructions. RNA quality was assessed using an Agilent BioAnalyzer 2100.
|
Label |
Cy5
|
Label protocol |
RNA samples were sent to Exiqon A/S (Vedbaek, Denmark) where they were labeled using the miRCURY Hy3/Hy5 labeling kit.
|
|
|
|
Hybridization protocol |
Labeled RNA samples were hybridized on the miRCURY LNA Array (v11.0) along with a Hy5-labeled common reference, composed of a pool of equal quantities of all 4 samples.
|
Scan protocol |
Scans were performed and quantified by Exiqon A/S using GenePix software (Molecular Devices).
|
Data processing |
The data were exported to Microsoft Excel worksheets, log2-transformed, and analyzed. The normalization involved normalization with a global Lowess algorithm.
|
|
|
Submission date |
Jun 23, 2010 |
Last update date |
Jun 23, 2010 |
Contact name |
wenyuan wang |
E-mail(s) |
wywang@mit.edu
|
Organization name |
MIT
|
Department |
BCS
|
Lab |
Tsai
|
Street address |
43 vassar st
|
City |
cambridge |
State/province |
MA |
ZIP/Postal code |
02139 |
Country |
USA |
|
|
Platform ID |
GPL7723 |
Series (1) |
|