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Sample GSM559486 Query DataSets for GSM559486
Status Public on Jun 24, 2010
Title Sirt1KO#2
Sample type RNA
 
Channel 1
Source name Sirt1KO
Organism Mus musculus
Characteristics strain: C57BL/6
gender: Male
tissue: hippocampus
Extracted molecule total RNA
Extraction protocol Total hippocampul RNA was isolated using TRIzol (Invitrogen) followed by purification over a glass fiber filter column (mirVana; Ambion), according to manufacturers' instructions. RNA quality was assessed using an Agilent BioAnalyzer 2100.
Label Cy3
Label protocol RNA samples were sent to Exiqon A/S (Vedbaek, Denmark) where they were labeled using the miRCURY Hy3/Hy5 labeling kit.
 
Channel 2
Source name common reference (comprised of an equal mixture of all 4 experimental samples)
Organism Mus musculus
Characteristics strain: C57BL/6
gender: Male
tissue: hippocampus
Extracted molecule total RNA
Extraction protocol Total hippocampul RNA was isolated using TRIzol (Invitrogen) followed by purification over a glass fiber filter column (mirVana; Ambion), according to manufacturers' instructions. RNA quality was assessed using an Agilent BioAnalyzer 2100.
Label Cy5
Label protocol RNA samples were sent to Exiqon A/S (Vedbaek, Denmark) where they were labeled using the miRCURY Hy3/Hy5 labeling kit.
 
 
Hybridization protocol Labeled RNA samples were hybridized on the miRCURY LNA Array (v11.0) along with a Hy5-labeled common reference, composed of a pool of equal quantities of all 4 samples.
Scan protocol Scans were performed and quantified by Exiqon A/S using GenePix software (Molecular Devices).
Data processing The data were exported to Microsoft Excel worksheets, log2-transformed, and analyzed. The normalization involved normalization with a global Lowess algorithm.
 
Submission date Jun 23, 2010
Last update date Jun 23, 2010
Contact name wenyuan wang
E-mail(s) wywang@mit.edu
Organization name MIT
Department BCS
Lab Tsai
Street address 43 vassar st
City cambridge
State/province MA
ZIP/Postal code 02139
Country USA
 
Platform ID GPL7723
Series (1)
GSE22530 Sirt1-microRNA profile

Data table header descriptions
ID_REF
VALUE lowess normalized log2 ratio (Cy3/Cy5)

Data table
ID_REF VALUE
28309 -0.97
13177 0.22
33596 0.47
42538 -0.98
29802 0.19
28213 0.42
42641 0.11
4610 0.32
17527 -1.12
42866 0.06
32772 -0.44
4390 -0.12
29153 -0.67
46807 -0.39
10943 0.34
42891 -0.41
17896 -0.33
11023 0.25
10947 0.41
11062 0.08

Total number of rows: 658

Table truncated, full table size 6 Kbytes.




Supplementary file Size Download File type/resource
GSM559486.txt.gz 889.7 Kb (ftp)(http) TXT
Processed data included within Sample table

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