|
| Status |
Public on Jun 24, 2010 |
| Title |
Ctrl#1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
wild-type
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: Male
tissue: hippocampus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total hippocampul RNA was isolated using TRIzol (Invitrogen) followed by purification over a glass fiber filter column (mirVana; Ambion), according to manufacturers' instructions. RNA quality was assessed using an Agilent BioAnalyzer 2100.
|
| Label |
Cy3
|
| Label protocol |
RNA samples were sent to Exiqon A/S (Vedbaek, Denmark) where they were labeled using the miRCURY Hy3/Hy5 labeling kit.
|
| |
|
| Channel 2 |
| Source name |
common reference (comprised of an equal mixture of all 4 experimental samples)
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: Male
tissue: hippocampus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total hippocampul RNA was isolated using TRIzol (Invitrogen) followed by purification over a glass fiber filter column (mirVana; Ambion), according to manufacturers' instructions. RNA quality was assessed using an Agilent BioAnalyzer 2100.
|
| Label |
Cy5
|
| Label protocol |
RNA samples were sent to Exiqon A/S (Vedbaek, Denmark) where they were labeled using the miRCURY Hy3/Hy5 labeling kit.
|
| |
|
| |
| Hybridization protocol |
Labeled RNA samples were hybridized on the miRCURY LNA Array (v11.0) along with a Hy5-labeled common reference, composed of a pool of equal quantities of all 4 samples.
|
| Scan protocol |
Scans were performed and quantified by Exiqon A/S using GenePix software (Molecular Devices).
|
| Data processing |
The data were exported to Microsoft Excel worksheets, log2-transformed, and analyzed. The normalization involved normalization with a global Lowess algorithm.
|
| |
|
| Submission date |
Jun 23, 2010 |
| Last update date |
Jun 23, 2010 |
| Contact name |
wenyuan wang |
| E-mail(s) |
wywang@mit.edu
|
| Organization name |
MIT
|
| Department |
BCS
|
| Lab |
Tsai
|
| Street address |
43 vassar st
|
| City |
cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL7723 |
| Series (1) |
|
