|
Status |
Public on Oct 12, 2021 |
Title |
HS_Colon_1 |
Sample type |
SRA |
|
|
Source name |
HS
|
Organism |
Rattus norvegicus |
Characteristics |
tissue: Colon treatment: HS
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA of kidney cortex and colon was extracted using TRIzol reagent according to the manufacturer’s instructions. cDNA libraries were prepared by TruePrep® DNA Library Prep Kit V2 for Illumina (Vazyme, Nanjing, China). Index-labeled libraries sized at 250–1000 bp fragment length were recovered by using VANTS® DNA Clean Beads (Vazyme, Nanjing, China). RNA libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Data processing |
NEXTSEQ 500 (Illumina) and single-end 75-bp read flow cells were used in this study The quality of sequencing data was evaluated using FastQC (v0.11.9). Adapters and low-quality reads were trimmed by Trimmomatic (v0.39). All the remaining qualified reads were mapped to the Rattus norvegicus genome (Rnor_6.0) using HiSat2 (v2.1.0). Then, FeatureCounts (v1.6.5) was employed for gene quantification to build an expression matrix. Genome_build: Rattus norvegicus genome (Rnor_6.0) Supplementary_files_format_and_content: csv file include raw count values for each tissue
|
|
|
Submission date |
Sep 27, 2021 |
Last update date |
Oct 12, 2021 |
Contact name |
Ruifeng Ding |
E-mail(s) |
ruifengding@usst.edu.cn
|
Organization name |
Changzheng Hospital, Second Affiliated Hospital of Naval Medical University
|
Department |
Department of Anesthesiology
|
Street address |
No. 415 Fengyang Road, Huangpu District
|
City |
Shanghai |
ZIP/Postal code |
200003 |
Country |
China |
|
|
Platform ID |
GPL20084 |
Series (1) |
GSE184844 |
Calcitriol ameliorates damage in high-salt diet-induced hypertension: evidence of communication with the gut–kidney axis |
|
Relations |
BioSample |
SAMN21855681 |
SRA |
SRX12374146 |