|
| Status |
Public on Oct 15, 2021 |
| Title |
Treg_day0_rep5 |
| Sample type |
genomic |
| |
|
| Source name |
Treg_day0
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Treg
donor: Donor_27
time point (day): 0
production run: NA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated using AllPrep DNA/RNA Mini Kit (Qiagen) or Quick-DNA Microprep Kit (Zymo Research) according to manufacturer's instructions. Bisulfite conversion was performed with EZ DNA Methylation Kit (Zymo Research) or EZ DNA Methylation Gold Kit (Zymo Research).
|
| Label |
Cy3, Cy5
|
| Label protocol |
Infinium HD Methylation Assay Reference Guide.
|
| |
|
| Hybridization protocol |
Bisulfite converted gDNA was fragmented, hybridized, and stained according to the Infinium HD Methylation Assay Reference Guide.
|
| Scan protocol |
Bead arrays were scanned on the iScan.
|
| Data processing |
Idat files were processed using standard preprocessing steps with minfi (R/Bioconductor). Methylation values were quantile normalized and batch corrected using the ComBat function from the package sva (R/Bioconductor).
|
| |
|
| Submission date |
Oct 13, 2021 |
| Last update date |
Oct 16, 2021 |
| Contact name |
Julia Polansky |
| E-mail(s) |
julia.polansky-biskup@charite.de
|
| Organization name |
Charité - Universitätsmedizin Berlin
|
| Street address |
Augustenburger Platz 1
|
| City |
Berlin |
| ZIP/Postal code |
13353 |
| Country |
Germany |
| |
|
| Platform ID |
GPL21145 |
| Series (1) |
| GSE185854 |
Epigenetic signature induced by prolonged culture may impact therapeutic function of Tregs used in adoptive cell therapy |
|
