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Sample GSM5627755 Query DataSets for GSM5627755
Status Public on Oct 15, 2021
Title Orbital adipose/connective tissue_excised_rep3
Sample type RNA
 
Source name Orbital adipose/connective tissue from orbital decompression surgery
Organism Homo sapiens
Characteristics diagnosis: Thyroid-associated ophthalmopathy (TAO)
tissue: Orbital adipose/connective tissue
gender: Male
Stage: Stable period
Growth protocol Liquid nitrogen quick freezing, -80 degrees refrigerator storage.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted and purified using RNeasy micro kit and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100.
Label Cy3
Label protocol Total RNA was amplified and labeled by Low Input Quick Amp Labeling Kit, One-Color (Cat.# 5190-2305, Agilent Technologies, Santa Clara, CA, US), following the manufacturer’s instructions.
 
Hybridization protocol Each slide was hybridized with 1.65μg Cy3-labeled cRNA using Gene Expression Hybridization Kit (Cat.# 5188-5242, Agilent Technologies, Santa Clara, CA, US) in Hybridization Oven (Cat.# G2545A, Agilent Technologies, Santa Clara, CA, US), according to the manufacturer’s instructions. After 17 hours hybridization, slides were washed in staining dishes (Cat.# 121, Thermo Shandon, Waltham, MA, US) with Gene Expression Wash Buffer Kit (Cat.# 5188-5327, Agilent Technologies, Santa Clara, CA, US), followed the manufacturer’s instructions.
Scan protocol Slides were scanned by Agilent Microarray Scanner (Cat#G2565CA, Agilent Technologies, Santa Clara, CA, US) with default settings, Dye channel: Green, Scan resolution=3μm, PMT 100%, 20bit. Data were extracted with Feature Extraction software 10.7 (Agilent Technologies, Santa Clara, CA, US).
Description TAO 3
611331
Data processing Raw data were normalized by Quantile algorithm, limma packages in R.
After the original data were normalized with the limma package in the software R, the differential genes were screened using Fold-change (fold of expression difference) and T-test (Student's t-test) statistical methods, and the selection conditions were as follows: 1) Fold Change (linear) =< 0.5 or Fold Change (linear) >= 2. 2) T-test p-value <0.05 or <0.01.
 
Submission date Oct 14, 2021
Last update date Dec 30, 2021
Contact name Zifan Yue
E-mail(s) y459016247@126.com
Phone 15821673019
Organization name Shanghai Changzheng Hospital
Department Ophthalmology
Street address No. 415 Fengyang Road, Hangpu District
City Shanghai
ZIP/Postal code 200001
Country China
 
Platform ID GPL30862
Series (1)
GSE185952 For lncRNA, circRNA and mRNA sequencing of thyroid-associated ophthalmopathy patients and normal people

Data table header descriptions
ID_REF
VALUE Normalized value

Data table
ID_REF VALUE
A_22_P00001056 1.350717972
CUST_25733_PI448380708 6.103817958
CUST_38665_PI448380708 4.908205494
A_23_P409516 8.819719429
CUST_76915_PI448380708 6.960074915
CUST_33809_PI448380708 3.68348649
A_23_P34018 15.05626966
A_24_P188941 14.81402872
CUST_20427_PI448301858 8.536676236
CUST_18337_PI448380708 5.119774551
CUST_1104_PI448301858 4.916913686
CUST_15263_PI448380708 6.513954304
CUST_42174_PI448380708 9.27543741
A_33_P3279969 5.236906553
CUST_105349_PI448380708 6.929307908
CUST_16722_PI448380708 12.64058975
CUST_22908_PI448380708 6.846361573
CUST_24735_PI448380708 1.493052407
A_33_P3317431 12.8997083
CUST_65766_PI448380708 3.069247974

Total number of rows: 173079

Table truncated, full table size 5556 Kbytes.




Supplementary file Size Download File type/resource
GSM5627755_611331_258636010008_S001_GE1_1200_Jun14_1_3.txt.gz 24.5 Mb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table
Processed data are available on Series record

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