|
| Status |
Public on Apr 15, 2022 |
| Title |
T00275917 |
| Sample type |
RNA |
| |
|
| Source name |
kidney, Chlorothiazide 20 milligram per kilogram per day
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: kidney
Sex: male
age: 16 weeks
strain: WKY/Izm
individual: W310-22 W310-23
scandate: 2005-07-13
compound: Chlorothiazide
dose: 20
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tissues were obtained from male rats at 16 weeks old. Total RNA was extracted using RNeasy maxi kit (Qiagen).
|
| Label |
Cy5
|
| Label protocol |
All reagents were provided in the CodeLink Expression Assay Kit. cRNA synthesis was performed according to the manufacturer's instructions using 2 ug of total RNA and labeled with Cy5.
|
| |
|
| Hybridization protocol |
The fragmented cRNA was hybridized overnight at 37C in hybridization buffer in an Innova 4080 shaking incubator (New Brunswick, Edison, NJ) at 300 rpm.
|
| Scan protocol |
Images were captured on an Axon GenePix Scanner (Arlington, TX) using GenePix Pro 6.0 Software and were analyzed using CodeLink Expression Analysis Software.
|
| Data processing |
Expression values were globally normalized to the median expression value of the whole rat probes. Signal smaller than or equal to 0.23 is weak and such probes should be discarded.
|
| |
|
| Submission date |
Nov 06, 2021 |
| Last update date |
Apr 15, 2022 |
| Contact name |
Fumihiko Takeuchi |
| E-mail(s) |
fumihiko@takeuchi.name
|
| Organization name |
National Center for Global Health and Medicine
|
| Department |
Research Institute
|
| Street address |
1-21-1 Toyama
|
| City |
Shinjuku-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
162-8655 |
| Country |
Japan |
| |
|
| Platform ID |
GPL2896 |
| Series (2) |
| GSE188349 |
Transcriptomic response in the heart and kidney to different types of antihypertensive drug administration [kidney] |
| GSE188350 |
Transcriptomic response in the heart and kidney to different types of antihypertensive drug administration |
|
