|
| Status |
Public on Jul 20, 2010 |
| Title |
Prostate cancer 50 Normal tissue |
| Sample type |
RNA |
| |
|
| Source name |
Prostate cancer radical prostatectomy specimen 50
|
| Organism |
Homo sapiens |
| Characteristics |
specimen: 50
disease state: normal control
|
| Treatment protocol |
Primary prostate cancer samples from thus far untreated patients after radical prostatectomy.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared by proteinase K digestion of formalin-fixed tissue samples followed by RNA precipitation. Epicentre MasterPure Complete DNA & RNA Purification Kit was used according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
FlashTag RNA Labeling with Genisphere RNA Labeling Kit
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip miRNA Procedure (FluidicsScript 450-0003, Hyb. 48°C)
|
| Scan protocol |
Affymetrix-3000 7G
|
| Description |
MiRNA gene expression data from corresponding normal tissue
Normal * 50
Total RNA from FFPE tissue
|
| Data processing |
Data files were analyzed with Partek genomics suite (version 6.5) using RMA background correction and quartile normalization
|
| |
|
| Submission date |
Jul 20, 2010 |
| Last update date |
Jul 22, 2010 |
| Contact name |
Sven Wach |
| E-mail(s) |
sven.wach@uk-erlangen.de
|
| Organization name |
University Hospital, Friedrich-Alexander-University Erlangen-Nuernberg
|
| Department |
Dept. of Urology
|
| Lab |
Experimental Urology
|
| Street address |
Glueckstrasse 6
|
| City |
Erlangen |
| ZIP/Postal code |
91054 |
| Country |
Germany |
| |
|
| Platform ID |
GPL8786 |
| Series (1) |
| GSE23022 |
MicroRNA expression data from human prostate cancer |
|
