|
Status |
Public on Jul 20, 2010 |
Title |
Prostate cancer 49 Tumor tissue |
Sample type |
RNA |
|
|
Source name |
Prostate cancer radical prostatectomy specimen 49
|
Organism |
Homo sapiens |
Characteristics |
specimen: 49 disease state: Primary prostate cancer tumor stage: pT3a gleason score: 9
|
Treatment protocol |
Primary prostate cancer samples from thus far untreated patients after radical prostatectomy.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was prepared by proteinase K digestion of formalin-fixed tissue samples followed by RNA precipitation. Epicentre MasterPure Complete DNA & RNA Purification Kit was used according to the manufacturer's instructions
|
Label |
biotin
|
Label protocol |
FlashTag RNA Labeling with Genisphere RNA Labeling Kit
|
|
|
Hybridization protocol |
Affymetrix GeneChip miRNA Procedure (FluidicsScript 450-0003, Hyb. 48°C)
|
Scan protocol |
Affymetrix-3000 7G
|
Description |
MiRNA gene expression from prostate cancer tissue Tumor * 49 Total RNA from FFPE tissue
|
Data processing |
Data files were analyzed with Partek genomics suite (version 6.5) using RMA background correction and quartile normalization
|
|
|
Submission date |
Jul 20, 2010 |
Last update date |
Jul 22, 2010 |
Contact name |
Sven Wach |
E-mail(s) |
sven.wach@uk-erlangen.de
|
Organization name |
University Hospital, Friedrich-Alexander-University Erlangen-Nuernberg
|
Department |
Dept. of Urology
|
Lab |
Experimental Urology
|
Street address |
Glueckstrasse 6
|
City |
Erlangen |
ZIP/Postal code |
91054 |
Country |
Germany |
|
|
Platform ID |
GPL8786 |
Series (1) |
GSE23022 |
MicroRNA expression data from human prostate cancer |
|