|
| Status |
Public on Dec 22, 2010 |
| Title |
Bombyx mori Middle Silk Gland SAGE library |
| Sample type |
SAGE |
| Anchor |
NlaIII
|
| Tag Count |
27401 |
| Tag Length |
10 |
| |
|
| Source name |
Middle Silk Gland of Bombyx mori, fith instar, day 3
|
| Organism |
Bombyx mori |
| Characteristics |
organism: Bombyx mori
tissue: Middle Silk gland
strain: F1 hybrids of 200 and 300
stage: day 3, fifth instar
|
| Biomaterial provider |
Sericulture Unit, La Mulatière , France
|
| Treatment protocol |
none
|
| Growth protocol |
Animals fed on fresh mulberry leaves
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Tri-Reagent (Sigma, USA).
|
| |
|
| Description |
Total RNA extracted prior to oligo-dT mRNA extraction.
RNAs controlled for quality and quantity on a Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA, USA).
|
| Data processing |
Tags extracted from PSG library (3072 sequenced inserts) with the Sagenhaft software (Beissbarth et al., 2004) then identified with Identitag (Keime et al., 2004).
|
| |
|
| Submission date |
Jul 23, 2010 |
| Last update date |
Dec 22, 2010 |
| Contact name |
Pierre Marie Couble |
| E-mail(s) |
pierre.couble@univ-lyon1.fr
|
| Phone |
33 473 44 80 85
|
| Fax |
33 4 72 43 26 85
|
| URL |
http://www.cgmc.univ-lyon1.fr
|
| Organization name |
CNRS-University Lyon 1
|
| Department |
Biology - INSB
|
| Lab |
CGMC
|
| Street address |
16 rue Dubois
|
| City |
Villeurbanne |
| ZIP/Postal code |
69622 |
| Country |
France |
| |
|
| Platform ID |
GPL2095 |
| Series (1) |
| GSE23098 |
Bombyx mori Posterior and Median Silk Gland SAGE transcriptome |
|
