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Status |
Public on Dec 31, 2013 |
Title |
Cortical neurons-Llac-15h-Rep 2 |
Sample type |
RNA |
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Source name |
Murine primary cortical neurons
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Organism |
Mus musculus |
Characteristics |
strain: Swiss Albino mice age: Gestation day 15-16 tissue: Primary cortical neurons age of culture: Day 5 treatment: 0.1uM Lactacystin treatment time-point: 15h
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Treatment protocol |
Lactacystin (Sigma, Louis, MO) were freshly prepared in dimethyl sulfoxide upon usage to get a stock concentration of 1mM respectively. Desired concentrations were achieved through dilution with NB medium. Day 5 cultured murine cortical neurons were treated with 0.1uM and 1uM lactacystin (achieved via dilution with Neurobasal medium) over a time course of 5- 24h. Neuronal samples then collected for global gene expression analyses.
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Growth protocol |
Neocortical neurons (gestational days 15 or 16) obtained from foetal cortices of Swiss albino mice were used to prepare the primary cultures employing previous described procedures with modifications [Cheung NS, Beart PM, Pascoe CJ, John CA, Bernard O. Human Bcl-2 protects against AMPA receptor-mediated apoptosis. J Neurochem. 2000; 74: 1613-1620.].
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted and cleaned up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with E-gene HDA-GT12 genetic analyzer.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
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|
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Hybridization protocol |
Standard Illumina hybridization protocol with a hybridization duration of 17h.
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Scan protocol |
Standard Illumina scanning protocol with a pmt value=478
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Description |
Replicate 2 4173865027_G
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Data processing |
The data were normalised using median normalisation with GeneSpring Ver 7.3
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Submission date |
Jul 26, 2010 |
Last update date |
Dec 31, 2013 |
Contact name |
Minghui Jessica Chen |
Organization name |
Menzies Research Institute
|
Department |
Neuroscience group
|
Lab |
A/P Steve Cheung
|
Street address |
Menzies Research Institute, University of Tasmania, Private Bag 24
|
City |
Hobart |
State/province |
Tasmania |
ZIP/Postal code |
7001 |
Country |
Australia |
|
|
Platform ID |
GPL6103 |
Series (1) |
GSE23155 |
Comparative global transcriptomic profiling of low and high concentration of lactacystin-mediated neuronal death |
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