|
| Status |
Public on Dec 31, 2013 |
| Title |
Cortical neurons-Llac-24h-Rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Murine primary cortical neurons
|
| Organism |
Mus musculus |
| Characteristics |
strain: Swiss Albino mice
age: Gestation day 15-16
tissue: Primary cortical neurons
age of culture: Day 5
treatment: 0.1uM Lactacystin
treatment time-point: 24h
|
| Treatment protocol |
Lactacystin (Sigma, Louis, MO) were freshly prepared in dimethyl sulfoxide upon usage to get a stock concentration of 1mM respectively. Desired concentrations were achieved through dilution with NB medium. Day 5 cultured murine cortical neurons were treated with 0.1uM and 1uM lactacystin (achieved via dilution with Neurobasal medium) over a time course of 5- 24h. Neuronal samples then collected for global gene expression analyses.
|
| Growth protocol |
Neocortical neurons (gestational days 15 or 16) obtained from foetal cortices of Swiss albino mice were used to prepare the primary cultures employing previous described procedures with modifications [Cheung NS, Beart PM, Pascoe CJ, John CA, Bernard O. Human Bcl-2 protects against AMPA receptor-mediated apoptosis. J Neurochem. 2000; 74: 1613-1620.].
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted and cleaned up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with E-gene HDA-GT12 genetic analyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol with a hybridization duration of 17h.
|
| Scan protocol |
Standard Illumina scanning protocol with a pmt value=478
|
| Description |
Replicate 2
4173865027_H
|
| Data processing |
The data were normalised using median normalisation with GeneSpring Ver 7.3
|
| |
|
| Submission date |
Jul 26, 2010 |
| Last update date |
Dec 31, 2013 |
| Contact name |
Minghui Jessica Chen |
| Organization name |
Menzies Research Institute
|
| Department |
Neuroscience group
|
| Lab |
A/P Steve Cheung
|
| Street address |
Menzies Research Institute, University of Tasmania, Private Bag 24
|
| City |
Hobart |
| State/province |
Tasmania |
| ZIP/Postal code |
7001 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6103 |
| Series (1) |
| GSE23155 |
Comparative global transcriptomic profiling of low and high concentration of lactacystin-mediated neuronal death |
|
