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Sample GSM570194 Query DataSets for GSM570194
Status Public on Jan 10, 2011
Title Cortex-I/R-8h-Rep2_WT-MCAO
Sample type RNA
 
Source name Infarct Cortex
Organism Mus musculus
Characteristics strain: C57/Bl6
genotype/variation: wild-type
age: 8-10 weeks
treatment: ischemia/reperfusion (I/R) injury
treatment time-point: 8h
Treatment protocol WT mice underwent the mid-cerebral artery occlusion (MCAO) model of cerebral ischemia-reperfusion injury as previously mentioned (Connolly et al. 1996). In experiments examining biochemical end-points and infarct size, a 2-hour ischemic period was used. Reduction in cerebral blood flow upon intraluminal suture insertion was confirmed with laser Doppler. Control sham animals were subjected to the initial anesthetic and neck incision only. All animals were put back onto a 37°C heat pad post-surgery to recover from anesthesia. After 2-hour of ischemic period, the animals underwent reperfusion by withdrawal of the suture under anesthetic condition and return onto the heat pad until euthanized at the designated time-points of 2, 8 or 24 hours.
Growth protocol All animal procedures were approved by the Monash University Animal Ethics Committee. Male WT mice on a C57BL/6J background of 8–10 weeks were employed in this study. Gpx1-/- mice demonstrate no overt phenotype and there exist no differences in the cerebral vascular anatomy between the Gpx1-/- mice and their WT counterparts (Crack et al. 2001; de Haan et al. 1998).
Extracted molecule total RNA
Extraction protocol RNA was extracted and cleaned up with QIAGEN miRNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with E-gene HDA-GT12 genetic analyzer.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol with a hybridization duration of 17h.
Scan protocol Standard Illumina scanning protocol with a pmt value=478
Description Replicate 2
4421339019_G
Data processing The data were normalised using median normalisation with GeneSpring Ver 7.3
 
Submission date Jul 26, 2010
Last update date Jan 10, 2011
Contact name Minghui Jessica Chen
Organization name Menzies Research Institute
Department Neuroscience group
Lab A/P Steve Cheung
Street address Menzies Research Institute, University of Tasmania, Private Bag 24
City Hobart
State/province Tasmania
ZIP/Postal code 7001
Country Australia
 
Platform ID GPL6885
Series (2)
GSE23160 Global transcriptomic profiling of ischemic/reperfusion injury in an in vivo wild-type mouse model.
GSE23163 Global transcriptomic profiling of ischemic/reperfusion injury in an in vivo mouse model.

Data table header descriptions
ID_REF
VALUE Median normalisation, p<0.05

Data table
ID_REF VALUE
ILMN_1248788 0.7509455
ILMN_2707227 1.0618472
ILMN_2896528 0.9319147
ILMN_2721178 1.0195645
ILMN_1227723 1.0480806
ILMN_3033922 1.1792237
ILMN_3092673 1.130341
ILMN_2730714 0.9674055
ILMN_3162224 0.99726695
ILMN_2816356 0.82648695
ILMN_2808939 1.0735241
ILMN_2634564 1.1057281
ILMN_1216623 1.2201401
ILMN_1215157 1.298236
ILMN_1228777 0.9374295
ILMN_2737647 0.86562836
ILMN_1216425 0.7825125
ILMN_2734484 1.0581759
ILMN_2652961 0.820326
ILMN_2952292 0.96760386

Total number of rows: 25697

Table truncated, full table size 580 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Raw data are available on Series record

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