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Sample GSM570208 Query DataSets for GSM570208
Status Public on Jan 10, 2011
Title Striatum-I/R-2h-Rep4_WT-MCAO
Sample type RNA
Source name Infarct Striatum
Organism Mus musculus
Characteristics strain: C57/Bl6
genotype/variation: wild-type
age: 8-10 weeks
treatment: ischemia/reperfusion (I/R) injury
treatment time-point: 2h
Treatment protocol WT mice underwent the mid-cerebral artery occlusion (MCAO) model of cerebral ischemia-reperfusion injury as previously mentioned (Connolly et al. 1996). In experiments examining biochemical end-points and infarct size, a 2-hour ischemic period was used. Reduction in cerebral blood flow upon intraluminal suture insertion was confirmed with laser Doppler. Control sham animals were subjected to the initial anesthetic and neck incision only. All animals were put back onto a 37°C heat pad post-surgery to recover from anesthesia. After 2-hour of ischemic period, the animals underwent reperfusion by withdrawal of the suture under anesthetic condition and return onto the heat pad until euthanized at the designated time-points of 2, 8 or 24 hours.
Growth protocol All animal procedures were approved by the Monash University Animal Ethics Committee. Male WT mice on a C57BL/6J background of 8–10 weeks were employed in this study. Gpx1-/- mice demonstrate no overt phenotype and there exist no differences in the cerebral vascular anatomy between the Gpx1-/- mice and their WT counterparts (Crack et al. 2001; de Haan et al. 1998).
Extracted molecule total RNA
Extraction protocol RNA was extracted and cleaned up with QIAGEN miRNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with E-gene HDA-GT12 genetic analyzer.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol with a hybridization duration of 17h.
Scan protocol Standard Illumina scanning protocol with a pmt value=478
Description Replicate 4
Data processing The data were normalised using median normalisation with GeneSpring Ver 7.3
Submission date Jul 26, 2010
Last update date Jan 10, 2011
Contact name Minghui Jessica Chen
Organization name Menzies Research Institute
Department Neuroscience group
Lab A/P Steve Cheung
Street address Menzies Research Institute, University of Tasmania, Private Bag 24
City Hobart
State/province Tasmania
ZIP/Postal code 7001
Country Australia
Platform ID GPL6885
Series (2)
GSE23160 Global transcriptomic profiling of ischemic/reperfusion injury in an in vivo wild-type mouse model.
GSE23163 Global transcriptomic profiling of ischemic/reperfusion injury in an in vivo mouse model.

Data table header descriptions
VALUE Median normalisation, p<0.05

Data table
ILMN_1248788 1.2940006
ILMN_2707227 1.0817317
ILMN_2896528 0.66374665
ILMN_2721178 0.79173505
ILMN_1227723 0.80934525
ILMN_3033922 0.8175312
ILMN_3092673 0.8586773
ILMN_2730714 0.73058605
ILMN_3162224 0.6864093
ILMN_2816356 1.0020014
ILMN_2808939 1.0697719
ILMN_2634564 0.8729946
ILMN_1216623 0.85045135
ILMN_1215157 0.76339144
ILMN_1228777 1.0142143
ILMN_2737647 1.0610229
ILMN_1216425 1.2226042
ILMN_2734484 0.6923354
ILMN_2652961 1.1268017
ILMN_2952292 1.0696009

Total number of rows: 25697

Table truncated, full table size 579 Kbytes.

Supplementary data files not provided
Processed data included within Sample table
Raw data are available on Series record

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