|
| Status |
Public on Jan 10, 2011 |
| Title |
Cortex-I/R-24h-Rep4_Gpx1-/- |
| Sample type |
RNA |
| |
|
| Source name |
Infarct Cortex
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/Bl6
genotype/variation: Gpx1-/-
treatment: ischemia/reperfusion (I/R) injury
age: 8-10 weeks
treatment time-point: 24h
|
| Treatment protocol |
Gpx1-/- mice underwent the mid-cerebral artery occlusion (MCAO) model of cerebral ischemia-reperfusion injury as previously mentioned (Connolly et al. 1996). In experiments examining biochemical end-points and infarct size, a 2-hour ischemic period was used. Reduction in cerebral blood flow upon intraluminal suture insertion was confirmed with laser Doppler. Control sham animals were subjected to the initial anesthetic and neck incision only. All animals were put back onto a 37°C heat pad post-surgery to recover from anesthesia. After 2-hour of ischemic period, the animals underwent reperfusion by withdrawal of the suture under anesthetic condition and return onto the heat pad until euthanized at the designated time-points of 2, 8 or 24 hours.
|
| Growth protocol |
All animal procedures were approved by the Monash University Animal Ethics Committee. Male WT mice on a C57BL/6J background of 8–10 weeks were employed in this study. Gpx1-/- mice demonstrate no overt phenotype and there exist no differences in the cerebral vascular anatomy between the Gpx1-/- mice and their WT counterparts (Crack et al. 2001; de Haan et al. 1998).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted and cleaned up with QIAGEN miRNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with E-gene HDA-GT12 genetic analyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol with a hybridization duration of 17h.
|
| Scan protocol |
Standard Illumina scanning protocol with a pmt value=478
|
| Description |
Replicate 4
4421339060_H
|
| Data processing |
The data were normalised using median normalisation with GeneSpring Ver 7.3
|
| |
|
| Submission date |
Jul 26, 2010 |
| Last update date |
Jan 10, 2011 |
| Contact name |
Minghui Jessica Chen |
| Organization name |
Menzies Research Institute
|
| Department |
Neuroscience group
|
| Lab |
A/P Steve Cheung
|
| Street address |
Menzies Research Institute, University of Tasmania, Private Bag 24
|
| City |
Hobart |
| State/province |
Tasmania |
| ZIP/Postal code |
7001 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE23162 |
Global transcriptomic profiling of ischemic/reperfusion injury in an in vivo Gpx1 -/- transgenic mouse model. |
| GSE23163 |
Global transcriptomic profiling of ischemic/reperfusion injury in an in vivo mouse model. |
|
