|
| Status |
Public on Jul 10, 2013 |
| Title |
Pituitary lesion, homozygous for Cdknb1 mutation, biological replicate 02 |
| Sample type |
RNA |
| |
|
| Source name |
Pituitary lesion, sample 08/666.2
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague–Dawley white eye/Wistar–Kyoto
age: 11 months
gender: Female
genotype/variation: p27Kip1 (Cdknb1) mutant, homozygous
tissue: pituitary lesion
|
| Growth protocol |
Animals per housed under spf conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen) including DNase treatment
|
| Label |
biotin
|
| Label protocol |
GeneChip® Whole Transcript (WT) Sense Target Labeling Assay (Affymetrix)
|
| |
|
| Hybridization protocol |
According to the Affymetrix expression protocol (HWS kit)
|
| Scan protocol |
According to the Affymetrix expression protocol
|
| Description |
Gene expression data from pituitary lesions
|
| Data processing |
RMA probeset summary and quantile normalisation (Expression console)
|
| |
|
| Submission date |
Jul 27, 2010 |
| Last update date |
Dec 18, 2013 |
| Contact name |
Johannes Beckers |
| E-mail(s) |
johannes.beckers@helmholtz-munich.de
|
| Organization name |
Helmholtz Zentrum Muenchen
|
| Department |
Institute of Experimental Genetics
|
| Street address |
Ingolstaedter Landstr. 1
|
| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE23207 |
Transcriptome analysis of MENX‑associated rat pituitary adenomas identifies novel molecular mechanisms involved in the pathogenesis of human pituitary gonadotroph adenomas |
|
