|
| Status |
Public on Mar 02, 2011 |
| Title |
Sua5B cells infected with Wolbachia strain wRi from Drosophila simulans, replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Anopheles gambiae Sua5B cells
|
| Organism |
Anopheles gambiae |
| Characteristics |
infection: Wolbachia strain wRi from Drosophila simulans
|
| Treatment protocol |
Either wAlbB or wRi were transferred into Sua5B cells then passaged for over 30 generations before microarray analysis.
|
| Growth protocol |
Anopheles gambiae Sua5B cells were cultured in Scnhieder's medium at room tempreture.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Homogenization and lysis of cells with Lysing Matrix D (Qbiogene) in Trizol LS (Invitrogen) with FastPrep120 (Qbiogene) instrument. Purification with Qiagen RNeasy Mini kit. All performed according to the manufacturers' instructions.
|
| Label |
biotin
|
| Label protocol |
5 ug total RNA was processed according to the Affymetrix recommended protocol for Eukaryotic samples, Affymetrix technical manual, revison 5.
|
| |
|
| Hybridization protocol |
Fragmented, biotinylated cRNA was hybridized to Plasmodium/Anoptheles GeneChips for 16hours at 45oC and 60rpm. Affymetrix recommended signal amplifcation protocol for washing/staining of Eukaryotic targets was performed in a FS450 automated fluidics station.
|
| Scan protocol |
GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
|
| Description |
wRI strain Wolbachia infection gene expression data
|
| Data processing |
The data were analyzed with Partek Genomics Suite software, version 6.4. GC-RMA algorithm defaults were used for background correction (GC-RMA), normalization (Quantile), and summarization (median polish) of probesets
|
| |
|
| Submission date |
Jul 28, 2010 |
| Last update date |
Mar 02, 2011 |
| Contact name |
Grant Hughes |
| E-mail(s) |
ghughes@jhsph.edu
|
| Organization name |
Johns Hopkins
|
| Department |
Bloomberg School of Public Health
|
| Lab |
Rasgon
|
| Street address |
615 N Wolfe St
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL1321 |
| Series (1) |
| GSE23215 |
Microarray analysis of Wolbachia infected Anopheles gambiae Sua5B cells |
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