NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM572468 Query DataSets for GSM572468
Status Public on Nov 16, 2010
Title 10M rep2
Sample type RNA
 
Source name PGC 10M dpp
Organism Mus musculus
Characteristics gender: male
age: 10 days
cell type: primordial germ cells
Extracted molecule total RNA
Extraction protocol 300 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 12h).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
 
Hybridization protocol Hybridized for 18 hrs onto MouseRef-8 v2 gene expression BeadChips (Illumina) following the manufacturer's instructions.
Scan protocol Chips were scanned using the iScan reader and accompanying software.
Description Replicate 2
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was performed using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
 
Submission date Jul 29, 2010
Last update date Jan 15, 2022
Contact name Marcos J. Araúzo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Phone +34 943 00 6108
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bionformatics
Street address Rogentstrasse
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6885
Series (1)
GSE23322 Identification of genes specific to mouse primordial germ cells through dynamic global gene expression

Data table header descriptions
ID_REF
VALUE Quantile-normalized signal

Data table
ID_REF VALUE
ILMN_1212607 2.243446
ILMN_1212612 1.989898
ILMN_1212619 5.723925
ILMN_1212628 1.766911
ILMN_1212632 5.262809
ILMN_1212636 10.845471
ILMN_1212637 8.450973
ILMN_1212645 2.005140
ILMN_1212648 6.931194
ILMN_1212653 2.881410
ILMN_1212672 7.340705
ILMN_1212682 3.568364
ILMN_1212683 1.452833
ILMN_1212685 1.765817
ILMN_1212692 4.300937
ILMN_1212693 7.651875
ILMN_1212695 2.302615
ILMN_1212698 1.478583
ILMN_1212702 6.922300
ILMN_1212703 3.483309

Total number of rows: 25697

Table truncated, full table size 553 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap