NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM573573 Query DataSets for GSM573573
Status Public on Aug 03, 2010
Title zebrafish_han_WT_Rep1
Sample type RNA
 
Source name hand2 wild-type sibling,19hpf
Organism Danio rerio
Characteristics stage: 19 hpf
tissue: Whole embryo
Growth protocol Embryos were collected, grown in blue water (60 mg/mL Instant Ocean and 0.025% Methylene blue) at 28.5 degrees Celcius and dechorionated prior to RNA extraction
Extracted molecule total RNA
Extraction protocol 3-9mg of total RNA from 10 to 25 embryos per sample were isolated using the RNeasy kit (Qiagen) according to manufacturer's instructions.
Label biotin
Label protocol ambion megascript labeling, with biotin labeled dNTP
 
Hybridization protocol Affymetrix standard protocol
Scan protocol According to manufacturer
Data processing MAS5, overall intensity was targetted to 500
 
Submission date Aug 02, 2010
Last update date Aug 02, 2010
Contact name Zayra Viviana Garavito-Aguilar
E-mail(s) zgaravitoaguilar@ucsd.edu
Fax (858)534-0555
Organization name University of California, San Diego
Department Division of Biological Sciences
Lab Yelon Lab
Street address 9500 Gilman Drive #0347
City La Jolla
State/province CA
ZIP/Postal code 92093-0347
Country USA
 
Platform ID GPL1319
Series (1)
GSE23381 Transcription profile of zebrafish hand2 (hanS6 allele) mutant embryos at 19hpf

Data table header descriptions
ID_REF
VALUE GCOS signal intensity
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-3_at 142.48727 P 0.003212301
AFFX-BioB-5_at 154.30534 P 6.62E-04
AFFX-BioB-M_at 179.35193 P 5.17E-05
AFFX-BioC-3_at 349.27377 P 4.43E-05
AFFX-BioC-5_at 414.78815 P 7.01E-05
AFFX-BioDn-3_at 3062.1775 P 6.02E-05
AFFX-BioDn-5_at 1037.0416 P 4.43E-05
AFFX-CreX-3_at 10347.749 P 4.43E-05
AFFX-CreX-5_at 6654.115 P 4.43E-05
AFFX-DapX-3_at 7.2808785 A 0.7723636
AFFX-DapX-5_at 14.898433 A 0.27514598
AFFX-DapX-M_at 23.760324 A 0.123572305
AFFX-Dr-AB076373-1_at 36.416164 A 0.6604422
AFFX-Dr-acta1-3_at 9784.127 P 2.19E-04
AFFX-Dr-acta1-5_at 987.39136 P 0.0078125
AFFX-Dr-acta1-5_x_at 2214.743 P 2.19E-04
AFFX-Dr-acta1-M_at 4869.263 P 2.19E-04
AFFX-Dr-AF292559-1_at 18.686516 A 0.47938037
AFFX-Dr-AF292559-2_s_at 0.6554993 A 0.56163913
AFFX-Dr-AF292559-3_s_at 2.0993135 A 0.910595

Total number of rows: 15617

Table truncated, full table size 588 Kbytes.




Supplementary file Size Download File type/resource
GSM573573.CEL.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap